Differentiation occupies a foundational position across the depth-psychology corpus, operating simultaneously as a cosmological principle, a developmental process, and a structural prerequisite for psychological health. In Jung’s Gnostic-inflected cosmology, as rendered in the Seven Sermons and the Red Book, differentiation is nothing less than the ontological ground of created existence: where the Pleroma is undifferentiated totality, the individual human being is constitutively differentiated, and consciousness itself is the activity of differentiating. This metaphysical register is complemented by the typological register, where differentiation names the process by which superior and auxiliary functions crystallize out of the undifferentiated psychic matrix — a process Beebe and Myers extend into the practical grammar of type development. Neumann situates differentiation within the phylogenetic arc of consciousness, tracing its emergence as the decisive event separating individual ego from collective participation mystique. Siegel imports the term into interpersonal neurobiology, where it denotes the necessary precondition for integration: components must first be differentiated before they can be meaningfully linked. Maté employs it clinically, diagnosing addiction as a failure of self-differentiation. Simondon, at the philosophical frontier, treats integration and differentiation as twin operators of transduction in living systems. The corpus thus reveals a term that is equally at home in Gnostic speculation, developmental theory, clinical diagnosis, and cognitive neuroscience — a measure of its structural indispensability to any serious psychology of individuation.